As indicated by his parents, Ambati was doing math at 4. years old At age 11, he moved on from Baltimore City College and co-created an exploration book on AIDS[citation needed].He moved on from New York University at 13 years old. He moved on from Mount Sinai School of Medicine with unique excellence at 17 years old, scoring above 99 percent on his National Medical Boards and turning into the world's most youthful specialist in 1995. Ambati communicated that he loathed being contrasted with Doogie Howser, the anecdotal adolescent doctor. Ambati portrayed himself as being prominent with individuals and, standing 6 feet tall, did not show up excessively youthful for a vocation in medicine — when he entered therapeutic school at 14 years of age, some expected that he was one of the consistent restorative students. 

He finished an ophthalmology residency at Harvard University, where he created procedures to turn around corneal angiogenesis in the wake of turning into a victor at the Westinghouse Science Talent Search and the International Science and Engineering Fair and turning into a National Merit Scholar.[citation needed] He got the prestigious Raja-Lakshmi Award in the year 1995 from Sri Raja-Lakshmi Foundation, Chennai. Subsequent to finishing an association in cornea and refractive surgery at Duke University in 2002, he joined the personnel of the Medical College of Georgia, where he honed clinical ophthalmology and directed examination in such zones as corneal angiogenesis and results of corneal and refractive surgery. He likewise volunteers with the ORBIS Flying Eye Hospital, flying out to under-favored nations to practice and educate ophthalmic surgery. As of now, Dr. Ambati is an educator of ophthalmology and visual sciences, assistant partner teacher of neurobiology and life structures, and executive of corneal exploration at the University of Utah School of Medicine.